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KMID : 0364820120480020116
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Korean Journal of Microbiology
2012 Volume.48 No. 2 p.116 ~ p.124
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Antibiotics Resistance and Molecular Analysis of Enterococcus Isolated from the Han-river in Korea
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Kwon Oh-Min
Kim Mal-Nam
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Abstract
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Identification was performed in March 2008 for the 76 Enterococcus strains isolated from the Han-river, which is
used as water supply for Seoul citizens. The antibiotic susceptibility, antibiotic resistant structural analysis,
trans-conjugation, pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were also
carried out for the isolated strains. Among the isolated strains, 25 strains were E. casseliflavus, 4 strains were E.
faecalis and 1 strain was E. hirae. Investigation of antibiotic susceptibility indicated that 15 strains demonstrated
tolerance against vancomycin, and that 11 strains of E. faecium and 4 strains of E. casseliflavus were VRE. The vanA
gene detection of the VRE strains revealed that 6 E. faecium strains were vancomycin-resistant Enterococcus
faecium (VREF) possessing vanA. Analyses of transposon Tn1546 structure containing vanA demonstrated that
Km36 and Km37 belonged to Tn1 type, Km20 and Km38 was Tn2 type, and Km39 and Km40 was Tn3 type. PFGE
disclosed that among the 6 VREF strains, Km36 and Km37 exhibited equivalent subtype, while the rest 4 strains
showed subtypes different to each other. MLST for the 6 VREF strains disclosed that 3 strains were ST78, while the
rest 3 strains were ST18, ST192 and ST230, respectively. All these clonal complexes were derived from CC17 which
has been isolated from clinical sources. 4 strains belonged to CC78, while the rest 2 strains were CC18 and CC192,
respectively
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KEYWORD
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Enterococcus, Tn1546, vanA, multilocus sequence typing, vancomycin
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